Engineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato
نویسندگان
چکیده
منابع مشابه
Phenotypic and Molecular Screening of Tomato Germplasm for Resistance to Tomato Yellow Leaf Curl Virus
Tomato yellow leaf curl virus (TYLCV) is a major tomato virus in tropical and subtropical regions. In this study, 134 accessions of Solanum lycopersicum and six accessions of Solanum peruvianum were assessed for resistance to an Iranian isolate of TYLCV. Plants were inoculated using whiteflies (Bemisia tabaci) and the reaction of plants was evaluated based on either disease symptoms or viral DN...
متن کاملNatural recombination between Tomato yellow leaf curl virus-is and Tomato leaf curl virus.
The complete genome sequences (2791 and 2793 nt) of isolates of Tomato yellow leaf curl virus-Is (TYLCV-Is) from Spain (SP72/97) and Portugal (Port2/95) were determined. These isolates are closely related to TYLCV-Is isolates reported in Japan (Japan-A and Japan-S) and Israel (Israel/Mild). Comparison of all sequenced isolates of TYLCV-Is showed that part of the genome comprising the intergenic...
متن کاملPyramiding of genes conferring resistance to Tomato yellow leaf curl virus from different wild tomato species
Tomato (Solanum lycopersicum) production in tropical and subtropical regions of the world is limited by the endemic presence of Tomato yellow leaf curl virus (TYLCV). Breeding programmes aimed at producing TYLCV-resistant tomato cultivars have utilized resistance sources derived from wild tomato species. So far, all reported breeding programmes have introgressed TYLCV resistance from a single w...
متن کاملphenotypic and molecular screening of tomato germplasm for resistance to tomato yellow leaf curl virus
tomato yellow leaf curl virus (tylcv) is a major tomato virus in tropical and subtropical regions. in this study, 134 accessions of solanum lycopersicum and six accessions of solanum peruvianum were assessed for resistance to an iranian isolate of tylcv. plants were inoculated using whiteflies (bemisia tabaci) and the reaction of plants was evaluated based on either disease symptoms or viral dn...
متن کاملTransgenic tobacco cells: a permissive system for the assessment of resistance strategies against Tomato yellow leaf curl virus
Tomato yellow leaf curl virus (TYLCV) is a major threat to tomato production in the tropics and sub-tropics around the world. The application of genetic engineering and pathogen derived resistance mechanisms to obtain tomatoes that are resistant to this pathogen is considered a promising alternative to the current protective practice against the virus. However, the development of transgenic tom...
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ژورنال
عنوان ژورنال: Plant Signaling & Behavior
سال: 2018
ISSN: 1559-2324
DOI: 10.1080/15592324.2018.1525996